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| Jouault, Albane; Gobet, Angelique; Simon, Marjolaine; Portier, Emilie; Perennou, Morgan; Corre, Erwan; Gaillard, Fanny; Vallenet, David; Michel, Gurvan; Fleury, Yannick; Bazire, Alexis; Dufour, Alain. |
| The aim was to identify and study the antibiofilm protein secreted by the marine bacterium Pseudoalteromonas sp. 3J6. The latter is active against marine and terrestrial bacteria, including Pseudomonas aeruginosa clinical strains forming different biofilm types. Several amino acid sequences were obtained from the partially purified antibiofilm protein, named alterocin. The Pseudoalteromonas sp. 3J6 genome was sequenced and a candidate alt gene was identified by comparing the genome-encoded proteins to the sequences from purified alterocin. Expressing the alt gene in another non-active Pseudoalteromonas sp. strain, 3J3, demonstrated that it is responsible for the antibiofilm activity. Alterocin is a 139-residue protein including a predicted 20-residue... |
| Tipo: Text |
Palavras-chave: Antibiofilm protein; Pseudoalteromonas; Pseudomonas aeruginosa; Biofilm. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00643/75517/76405.pdf |
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| Gobet, Angelique; Barbeyron, Tristan; Matard-mann, Maria; Magdelenat, Ghislaine; Vallenet, David; Duchaud, Eric; Michel, Gurvan. |
| About half of seaweed biomass is composed of polysaccharides. Most of these complex polymers have a marked polyanionic character. For instance, the red algal cell wall is mainly composed of sulfated galactans, agars and carrageenans, while brown algae contain alginate and fucose-containing sulfated polysaccharides (FCSP) as cell wall polysaccharides. Some marine heterotrophic bacteria have developed abilities to grow on such macroalgal polysaccharides. This is the case of Pseudoalteromonas carrageenovora 9(T) (ATCC 43555(T)), a marine gammaproteobacterium isolated in 1955 and which was an early model organism for studying carrageenan catabolism. We present here the genomic analysis of P. carrageenovora. Its genome is composed of two chromosomes and of a... |
| Tipo: Text |
Palavras-chave: Carrageenan; CAZymes; Alginate; Gammaproteobacteria; Pseudoalteromonas; Marine bacteria; Algal holobiont; Biofilm. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00489/60070/63377.pdf |
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